The Isthmus of Corinth (Greek: Ισθμός της Κορίνθου) is the narrow land bridge which connects the Peloponnese peninsula with the rest of the mainland of Greece, near the city of Corinth. The Isthmus was known in the ancient world as the landmark separating the Peloponnese from mainland Greece. In the first century AD the geographer Strabo noted a stele on the Isthmus of Corinth, which bore two inscriptions. One towards the East, i.e. towards Megara, reading: "Here is not Peloponnesus, but Ionia" (τάδ᾽ οὐχὶ Πελοπόννησος, ἀλλ᾽ Ἰωνία) and the one towards the West, i.e. towards the Peloponnese: "Here is Peloponnesus, not Ionia" (τάδ᾽ ἐστὶ Πελοπόννησος, οὐκ Ἰωνία); Plutarch ascribed the erection of the stele to the Attic hero Theseus, on his way to Athens.

To the west of the Isthmus is the Gulf of Corinth, and to the east the Saronic Gulf. Since 1893 the Corinth Canal has run through the 6.3 km wide isthmus, effectively making the Peloponnese an island. Today, two road bridges, two railway bridges and two submersible bridges at both ends of the canal connect the mainland side of the isthmus with the Peloponnese side. Also a military emergency bridge is located at the west end of the canal.